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tlr 7 8 agonist r848  (InvivoGen)


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    InvivoGen tlr 7 8 agonist r848
    Tlr 7 8 Agonist R848, supplied by InvivoGen, used in various techniques. Bioz Stars score: 99/100, based on 2407 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/tlr+7+8+agonist/R848/pm41693562-66-10-16
    Average 99 stars, based on 2407 article reviews
    tlr 7 8 agonist r848 - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Infection:

    Article Title: Peculiar transcriptional reprogramming with functional impairment of dendritic cells upon exposure to transformed HTLV-1-infected cells
    Article Snippet: Toll-like receptor (TLR)-4 agonist (LPS, tlrl-3pelps; 1μg/mL) and TLR-7/8 agonist (R848, tlrl-r848; 3μg/mL) were purchased from Invivogen.. MeV IC323-eGFP [ ] is a recombinant MeV expressing the gene-encoding eGFP (using the plasmid-encoding MeV IC323-eGFP kindly provided by Yanagi, Kyushu University, Fukuoka, Japan).MeV IC323-eGFP [ ] is a recombinant MeV expressing the gene-encoding eGFP (using the plasmid-encoding MeV IC323-eGFP kindly provided by Yanagi, Kyushu University, Fukuoka, Japan).

    Article Title: Induction of tolerogenicity following a molecular dialogue between HTLV-1-infected T cells and dendritic cells
    Article Snippet: Every experiment was repeated on MDDCs derived from independent blood donors.Every experiment was repeated on MDDCs derived from independent blood donors.. Reagents and antibodies Toll-like receptor (TLR)-4 agonist (LPS, tlrl-3pelps; 1µg/mL) and TLR-7/8 agonist (R848, tlrl-r848; 3µg/mL) were purchased from Invivogen.. MeV IC323-eGFP 48 is a recombinant MeV expressing the geneencoding eGFP (using the plasmid-encoding MeV IC323-eGFP kindly provided by Yanagi, Kyushu University, Fukuoka, Japan).MeV IC323-eGFP 48 is a recombinant MeV expressing the geneencoding eGFP (using the plasmid-encoding MeV IC323-eGFP kindly provided by Yanagi, Kyushu University, Fukuoka, Japan).

    Article Title: Lymph node dendritic cells harbor inducible replication-competent HIV despite years of suppressive ART
    Article Snippet: HIV peptides , JPT Peptide Technology , Customized.HIV peptides , JPT Peptide Technology , Customized.. TLR-7/8 agonist , InvivoGen , CL264.. SEB , Sigma-Aldrich , S4881.SEB , Sigma-Aldrich , S4881.

    Article Title: Bacille Calmette–Guérin vaccine reprograms human neonatal lipid metabolism in vivo and in vitro
    Article Snippet: (LPC) kit , MyBioSource , Cat#: MBS2700657. .. TLR 7/8 agonist , Invivogen , R848 (Resiquimod) Cat#: tlrl-r848. .. Methanol , Applied Biosystems, ThermoFisher , Cat#: 400470.

    Article Title: Dendritic cell maturation, but not type I interferon exposure, restricts infection by HTLV-1, and viral transmission to T-cells
    Article Snippet: Chlorpromazine (20 μM, resuspended in H 2 O), diphenyl iodonium chloride (10 μM, resuspended in DMSO); dynasore (100 μM, resuspended in DMSO), methyl-β-cyclodextrin (10 mM, resuspended in H 2 O), nystatin (270 μM, resuspended in DMSO), amiloride (375 μM, resuspended in H 2 O), latrunculin (2 μM, resuspended in DMSO), cytochalasin D (2 μM, resuspended in DMSO), genistein (200 μM, resuspended in MeOH), wortmanin (40 μM, resuspended in DMSO), rottlerin (25 μM, resuspended in EtOH), Gö6953 (1 μM, resuspended in DMSO), NSC23766 trihydrochloride (250 μM, resuspended in H 2 O) and IPA-3 (125 μM, resuspended in DMSO) were purchased from Sigma.cytochalasin D (2 μM, resuspended in DMSO), genistein (200 μM, resuspended in MeOH), wortmanin (40 μM, resuspended in DMSO), rottlerin (25 μM, resuspended in EtOH), Gö6953 (1 μM, resuspended in DMSO), NSC23766 trihydrochloride (250 μM, resuspended in H 2 O) and IPA-3 (125 μM, resuspended in DMSO) were purchased from Sigma. ... Toll like receptor (TLR)-2 agonist (PAM3CSK4; 1 μg/ml); TLR-5 agonist (Flagellin; 200 ng/ml); TLR-3 agonist (Poly(I:C); 10 μg/ml); TLR-4 agonist (LPS; 1 μg/ml); TLR-7/8 agonist (R848; 3 μg/ml) were purchased from Invivogen.. Recombinant IFN-α (500–2000 IU/ml) was purchased from Tebu-Bio.Recombinant IFN-α (500–2000 IU/ml) was purchased from Tebu-Bio.

    Flow Cytometry:

    Article Title: Peculiar transcriptional reprogramming with functional impairment of dendritic cells upon exposure to transformed HTLV-1-infected cells
    Article Snippet: Toll-like receptor (TLR)-4 agonist (LPS, tlrl-3pelps; 1μg/mL) and TLR-7/8 agonist (R848, tlrl-r848; 3μg/mL) were purchased from Invivogen.. MeV IC323-eGFP [ ] is a recombinant MeV expressing the gene-encoding eGFP (using the plasmid-encoding MeV IC323-eGFP kindly provided by Yanagi, Kyushu University, Fukuoka, Japan).MeV IC323-eGFP [ ] is a recombinant MeV expressing the gene-encoding eGFP (using the plasmid-encoding MeV IC323-eGFP kindly provided by Yanagi, Kyushu University, Fukuoka, Japan).

    Article Title: Induction of tolerogenicity following a molecular dialogue between HTLV-1-infected T cells and dendritic cells
    Article Snippet: Every experiment was repeated on MDDCs derived from independent blood donors.Every experiment was repeated on MDDCs derived from independent blood donors.. Reagents and antibodies Toll-like receptor (TLR)-4 agonist (LPS, tlrl-3pelps; 1µg/mL) and TLR-7/8 agonist (R848, tlrl-r848; 3µg/mL) were purchased from Invivogen.. MeV IC323-eGFP 48 is a recombinant MeV expressing the geneencoding eGFP (using the plasmid-encoding MeV IC323-eGFP kindly provided by Yanagi, Kyushu University, Fukuoka, Japan).MeV IC323-eGFP 48 is a recombinant MeV expressing the geneencoding eGFP (using the plasmid-encoding MeV IC323-eGFP kindly provided by Yanagi, Kyushu University, Fukuoka, Japan).

    Article Title: Lymph node dendritic cells harbor inducible replication-competent HIV despite years of suppressive ART
    Article Snippet: HIV peptides , JPT Peptide Technology , Customized.HIV peptides , JPT Peptide Technology , Customized.. TLR-7/8 agonist , InvivoGen , CL264.. SEB , Sigma-Aldrich , S4881.SEB , Sigma-Aldrich , S4881.

    Article Title: Bacille Calmette–Guérin vaccine reprograms human neonatal lipid metabolism in vivo and in vitro
    Article Snippet: (LPC) kit , MyBioSource , Cat#: MBS2700657. .. TLR 7/8 agonist , Invivogen , R848 (Resiquimod) Cat#: tlrl-r848. .. Methanol , Applied Biosystems, ThermoFisher , Cat#: 400470.

    Article Title: Dendritic cell maturation, but not type I interferon exposure, restricts infection by HTLV-1, and viral transmission to T-cells
    Article Snippet: Chlorpromazine (20 μM, resuspended in H 2 O), diphenyl iodonium chloride (10 μM, resuspended in DMSO); dynasore (100 μM, resuspended in DMSO), methyl-β-cyclodextrin (10 mM, resuspended in H 2 O), nystatin (270 μM, resuspended in DMSO), amiloride (375 μM, resuspended in H 2 O), latrunculin (2 μM, resuspended in DMSO), cytochalasin D (2 μM, resuspended in DMSO), genistein (200 μM, resuspended in MeOH), wortmanin (40 μM, resuspended in DMSO), rottlerin (25 μM, resuspended in EtOH), Gö6953 (1 μM, resuspended in DMSO), NSC23766 trihydrochloride (250 μM, resuspended in H 2 O) and IPA-3 (125 μM, resuspended in DMSO) were purchased from Sigma.cytochalasin D (2 μM, resuspended in DMSO), genistein (200 μM, resuspended in MeOH), wortmanin (40 μM, resuspended in DMSO), rottlerin (25 μM, resuspended in EtOH), Gö6953 (1 μM, resuspended in DMSO), NSC23766 trihydrochloride (250 μM, resuspended in H 2 O) and IPA-3 (125 μM, resuspended in DMSO) were purchased from Sigma. ... Toll like receptor (TLR)-2 agonist (PAM3CSK4; 1 μg/ml); TLR-5 agonist (Flagellin; 200 ng/ml); TLR-3 agonist (Poly(I:C); 10 μg/ml); TLR-4 agonist (LPS; 1 μg/ml); TLR-7/8 agonist (R848; 3 μg/ml) were purchased from Invivogen.. Recombinant IFN-α (500–2000 IU/ml) was purchased from Tebu-Bio.Recombinant IFN-α (500–2000 IU/ml) was purchased from Tebu-Bio.

    Staining:

    Article Title: Peculiar transcriptional reprogramming with functional impairment of dendritic cells upon exposure to transformed HTLV-1-infected cells
    Article Snippet: Toll-like receptor (TLR)-4 agonist (LPS, tlrl-3pelps; 1μg/mL) and TLR-7/8 agonist (R848, tlrl-r848; 3μg/mL) were purchased from Invivogen.. MeV IC323-eGFP [ ] is a recombinant MeV expressing the gene-encoding eGFP (using the plasmid-encoding MeV IC323-eGFP kindly provided by Yanagi, Kyushu University, Fukuoka, Japan).MeV IC323-eGFP [ ] is a recombinant MeV expressing the gene-encoding eGFP (using the plasmid-encoding MeV IC323-eGFP kindly provided by Yanagi, Kyushu University, Fukuoka, Japan).

    Article Title: Induction of tolerogenicity following a molecular dialogue between HTLV-1-infected T cells and dendritic cells
    Article Snippet: Every experiment was repeated on MDDCs derived from independent blood donors.Every experiment was repeated on MDDCs derived from independent blood donors.. Reagents and antibodies Toll-like receptor (TLR)-4 agonist (LPS, tlrl-3pelps; 1µg/mL) and TLR-7/8 agonist (R848, tlrl-r848; 3µg/mL) were purchased from Invivogen.. MeV IC323-eGFP 48 is a recombinant MeV expressing the geneencoding eGFP (using the plasmid-encoding MeV IC323-eGFP kindly provided by Yanagi, Kyushu University, Fukuoka, Japan).MeV IC323-eGFP 48 is a recombinant MeV expressing the geneencoding eGFP (using the plasmid-encoding MeV IC323-eGFP kindly provided by Yanagi, Kyushu University, Fukuoka, Japan).

    Article Title: Lymph node dendritic cells harbor inducible replication-competent HIV despite years of suppressive ART
    Article Snippet: HIV peptides , JPT Peptide Technology , Customized.HIV peptides , JPT Peptide Technology , Customized.. TLR-7/8 agonist , InvivoGen , CL264.. SEB , Sigma-Aldrich , S4881.SEB , Sigma-Aldrich , S4881.

    Article Title: Bacille Calmette–Guérin vaccine reprograms human neonatal lipid metabolism in vivo and in vitro
    Article Snippet: (LPC) kit , MyBioSource , Cat#: MBS2700657. .. TLR 7/8 agonist , Invivogen , R848 (Resiquimod) Cat#: tlrl-r848. .. Methanol , Applied Biosystems, ThermoFisher , Cat#: 400470.

    Article Title: Dendritic cell maturation, but not type I interferon exposure, restricts infection by HTLV-1, and viral transmission to T-cells
    Article Snippet: Chlorpromazine (20 μM, resuspended in H 2 O), diphenyl iodonium chloride (10 μM, resuspended in DMSO); dynasore (100 μM, resuspended in DMSO), methyl-β-cyclodextrin (10 mM, resuspended in H 2 O), nystatin (270 μM, resuspended in DMSO), amiloride (375 μM, resuspended in H 2 O), latrunculin (2 μM, resuspended in DMSO), cytochalasin D (2 μM, resuspended in DMSO), genistein (200 μM, resuspended in MeOH), wortmanin (40 μM, resuspended in DMSO), rottlerin (25 μM, resuspended in EtOH), Gö6953 (1 μM, resuspended in DMSO), NSC23766 trihydrochloride (250 μM, resuspended in H 2 O) and IPA-3 (125 μM, resuspended in DMSO) were purchased from Sigma.cytochalasin D (2 μM, resuspended in DMSO), genistein (200 μM, resuspended in MeOH), wortmanin (40 μM, resuspended in DMSO), rottlerin (25 μM, resuspended in EtOH), Gö6953 (1 μM, resuspended in DMSO), NSC23766 trihydrochloride (250 μM, resuspended in H 2 O) and IPA-3 (125 μM, resuspended in DMSO) were purchased from Sigma. ... Toll like receptor (TLR)-2 agonist (PAM3CSK4; 1 μg/ml); TLR-5 agonist (Flagellin; 200 ng/ml); TLR-3 agonist (Poly(I:C); 10 μg/ml); TLR-4 agonist (LPS; 1 μg/ml); TLR-7/8 agonist (R848; 3 μg/ml) were purchased from Invivogen.. Recombinant IFN-α (500–2000 IU/ml) was purchased from Tebu-Bio.Recombinant IFN-α (500–2000 IU/ml) was purchased from Tebu-Bio.

    In Vitro:

    Article Title: Peculiar transcriptional reprogramming with functional impairment of dendritic cells upon exposure to transformed HTLV-1-infected cells
    Article Snippet: Toll-like receptor (TLR)-4 agonist (LPS, tlrl-3pelps; 1μg/mL) and TLR-7/8 agonist (R848, tlrl-r848; 3μg/mL) were purchased from Invivogen.. MeV IC323-eGFP [ ] is a recombinant MeV expressing the gene-encoding eGFP (using the plasmid-encoding MeV IC323-eGFP kindly provided by Yanagi, Kyushu University, Fukuoka, Japan).MeV IC323-eGFP [ ] is a recombinant MeV expressing the gene-encoding eGFP (using the plasmid-encoding MeV IC323-eGFP kindly provided by Yanagi, Kyushu University, Fukuoka, Japan).

    Article Title: Induction of tolerogenicity following a molecular dialogue between HTLV-1-infected T cells and dendritic cells
    Article Snippet: Every experiment was repeated on MDDCs derived from independent blood donors.Every experiment was repeated on MDDCs derived from independent blood donors.. Reagents and antibodies Toll-like receptor (TLR)-4 agonist (LPS, tlrl-3pelps; 1µg/mL) and TLR-7/8 agonist (R848, tlrl-r848; 3µg/mL) were purchased from Invivogen.. MeV IC323-eGFP 48 is a recombinant MeV expressing the geneencoding eGFP (using the plasmid-encoding MeV IC323-eGFP kindly provided by Yanagi, Kyushu University, Fukuoka, Japan).MeV IC323-eGFP 48 is a recombinant MeV expressing the geneencoding eGFP (using the plasmid-encoding MeV IC323-eGFP kindly provided by Yanagi, Kyushu University, Fukuoka, Japan).

    Article Title: Lymph node dendritic cells harbor inducible replication-competent HIV despite years of suppressive ART
    Article Snippet: HIV peptides , JPT Peptide Technology , Customized.HIV peptides , JPT Peptide Technology , Customized.. TLR-7/8 agonist , InvivoGen , CL264.. SEB , Sigma-Aldrich , S4881.SEB , Sigma-Aldrich , S4881.

    Article Title: Bacille Calmette–Guérin vaccine reprograms human neonatal lipid metabolism in vivo and in vitro
    Article Snippet: (LPC) kit , MyBioSource , Cat#: MBS2700657. .. TLR 7/8 agonist , Invivogen , R848 (Resiquimod) Cat#: tlrl-r848. .. Methanol , Applied Biosystems, ThermoFisher , Cat#: 400470.

    Article Title: Dendritic cell maturation, but not type I interferon exposure, restricts infection by HTLV-1, and viral transmission to T-cells
    Article Snippet: Chlorpromazine (20 μM, resuspended in H 2 O), diphenyl iodonium chloride (10 μM, resuspended in DMSO); dynasore (100 μM, resuspended in DMSO), methyl-β-cyclodextrin (10 mM, resuspended in H 2 O), nystatin (270 μM, resuspended in DMSO), amiloride (375 μM, resuspended in H 2 O), latrunculin (2 μM, resuspended in DMSO), cytochalasin D (2 μM, resuspended in DMSO), genistein (200 μM, resuspended in MeOH), wortmanin (40 μM, resuspended in DMSO), rottlerin (25 μM, resuspended in EtOH), Gö6953 (1 μM, resuspended in DMSO), NSC23766 trihydrochloride (250 μM, resuspended in H 2 O) and IPA-3 (125 μM, resuspended in DMSO) were purchased from Sigma.cytochalasin D (2 μM, resuspended in DMSO), genistein (200 μM, resuspended in MeOH), wortmanin (40 μM, resuspended in DMSO), rottlerin (25 μM, resuspended in EtOH), Gö6953 (1 μM, resuspended in DMSO), NSC23766 trihydrochloride (250 μM, resuspended in H 2 O) and IPA-3 (125 μM, resuspended in DMSO) were purchased from Sigma. ... Toll like receptor (TLR)-2 agonist (PAM3CSK4; 1 μg/ml); TLR-5 agonist (Flagellin; 200 ng/ml); TLR-3 agonist (Poly(I:C); 10 μg/ml); TLR-4 agonist (LPS; 1 μg/ml); TLR-7/8 agonist (R848; 3 μg/ml) were purchased from Invivogen.. Recombinant IFN-α (500–2000 IU/ml) was purchased from Tebu-Bio.Recombinant IFN-α (500–2000 IU/ml) was purchased from Tebu-Bio.

    MANN-WHITNEY:

    Article Title: Peculiar transcriptional reprogramming with functional impairment of dendritic cells upon exposure to transformed HTLV-1-infected cells
    Article Snippet: Toll-like receptor (TLR)-4 agonist (LPS, tlrl-3pelps; 1μg/mL) and TLR-7/8 agonist (R848, tlrl-r848; 3μg/mL) were purchased from Invivogen.. MeV IC323-eGFP [ ] is a recombinant MeV expressing the gene-encoding eGFP (using the plasmid-encoding MeV IC323-eGFP kindly provided by Yanagi, Kyushu University, Fukuoka, Japan).MeV IC323-eGFP [ ] is a recombinant MeV expressing the gene-encoding eGFP (using the plasmid-encoding MeV IC323-eGFP kindly provided by Yanagi, Kyushu University, Fukuoka, Japan).

    Article Title: Induction of tolerogenicity following a molecular dialogue between HTLV-1-infected T cells and dendritic cells
    Article Snippet: Every experiment was repeated on MDDCs derived from independent blood donors.Every experiment was repeated on MDDCs derived from independent blood donors.. Reagents and antibodies Toll-like receptor (TLR)-4 agonist (LPS, tlrl-3pelps; 1µg/mL) and TLR-7/8 agonist (R848, tlrl-r848; 3µg/mL) were purchased from Invivogen.. MeV IC323-eGFP 48 is a recombinant MeV expressing the geneencoding eGFP (using the plasmid-encoding MeV IC323-eGFP kindly provided by Yanagi, Kyushu University, Fukuoka, Japan).MeV IC323-eGFP 48 is a recombinant MeV expressing the geneencoding eGFP (using the plasmid-encoding MeV IC323-eGFP kindly provided by Yanagi, Kyushu University, Fukuoka, Japan).

    Article Title: Lymph node dendritic cells harbor inducible replication-competent HIV despite years of suppressive ART
    Article Snippet: HIV peptides , JPT Peptide Technology , Customized.HIV peptides , JPT Peptide Technology , Customized.. TLR-7/8 agonist , InvivoGen , CL264.. SEB , Sigma-Aldrich , S4881.SEB , Sigma-Aldrich , S4881.

    Article Title: Bacille Calmette–Guérin vaccine reprograms human neonatal lipid metabolism in vivo and in vitro
    Article Snippet: (LPC) kit , MyBioSource , Cat#: MBS2700657. .. TLR 7/8 agonist , Invivogen , R848 (Resiquimod) Cat#: tlrl-r848. .. Methanol , Applied Biosystems, ThermoFisher , Cat#: 400470.

    Article Title: Dendritic cell maturation, but not type I interferon exposure, restricts infection by HTLV-1, and viral transmission to T-cells
    Article Snippet: Chlorpromazine (20 μM, resuspended in H 2 O), diphenyl iodonium chloride (10 μM, resuspended in DMSO); dynasore (100 μM, resuspended in DMSO), methyl-β-cyclodextrin (10 mM, resuspended in H 2 O), nystatin (270 μM, resuspended in DMSO), amiloride (375 μM, resuspended in H 2 O), latrunculin (2 μM, resuspended in DMSO), cytochalasin D (2 μM, resuspended in DMSO), genistein (200 μM, resuspended in MeOH), wortmanin (40 μM, resuspended in DMSO), rottlerin (25 μM, resuspended in EtOH), Gö6953 (1 μM, resuspended in DMSO), NSC23766 trihydrochloride (250 μM, resuspended in H 2 O) and IPA-3 (125 μM, resuspended in DMSO) were purchased from Sigma.cytochalasin D (2 μM, resuspended in DMSO), genistein (200 μM, resuspended in MeOH), wortmanin (40 μM, resuspended in DMSO), rottlerin (25 μM, resuspended in EtOH), Gö6953 (1 μM, resuspended in DMSO), NSC23766 trihydrochloride (250 μM, resuspended in H 2 O) and IPA-3 (125 μM, resuspended in DMSO) were purchased from Sigma. ... Toll like receptor (TLR)-2 agonist (PAM3CSK4; 1 μg/ml); TLR-5 agonist (Flagellin; 200 ng/ml); TLR-3 agonist (Poly(I:C); 10 μg/ml); TLR-4 agonist (LPS; 1 μg/ml); TLR-7/8 agonist (R848; 3 μg/ml) were purchased from Invivogen.. Recombinant IFN-α (500–2000 IU/ml) was purchased from Tebu-Bio.Recombinant IFN-α (500–2000 IU/ml) was purchased from Tebu-Bio.

    Recombinant:

    Article Title: Peculiar transcriptional reprogramming with functional impairment of dendritic cells upon exposure to transformed HTLV-1-infected cells
    Article Snippet: Toll-like receptor (TLR)-4 agonist (LPS, tlrl-3pelps; 1μg/mL) and TLR-7/8 agonist (R848, tlrl-r848; 3μg/mL) were purchased from Invivogen.. MeV IC323-eGFP [ ] is a recombinant MeV expressing the gene-encoding eGFP (using the plasmid-encoding MeV IC323-eGFP kindly provided by Yanagi, Kyushu University, Fukuoka, Japan).MeV IC323-eGFP [ ] is a recombinant MeV expressing the gene-encoding eGFP (using the plasmid-encoding MeV IC323-eGFP kindly provided by Yanagi, Kyushu University, Fukuoka, Japan).

    Article Title: Induction of tolerogenicity following a molecular dialogue between HTLV-1-infected T cells and dendritic cells
    Article Snippet: Every experiment was repeated on MDDCs derived from independent blood donors.Every experiment was repeated on MDDCs derived from independent blood donors.. Reagents and antibodies Toll-like receptor (TLR)-4 agonist (LPS, tlrl-3pelps; 1µg/mL) and TLR-7/8 agonist (R848, tlrl-r848; 3µg/mL) were purchased from Invivogen.. MeV IC323-eGFP 48 is a recombinant MeV expressing the geneencoding eGFP (using the plasmid-encoding MeV IC323-eGFP kindly provided by Yanagi, Kyushu University, Fukuoka, Japan).MeV IC323-eGFP 48 is a recombinant MeV expressing the geneencoding eGFP (using the plasmid-encoding MeV IC323-eGFP kindly provided by Yanagi, Kyushu University, Fukuoka, Japan).

    Article Title: Lymph node dendritic cells harbor inducible replication-competent HIV despite years of suppressive ART
    Article Snippet: HIV peptides , JPT Peptide Technology , Customized.HIV peptides , JPT Peptide Technology , Customized.. TLR-7/8 agonist , InvivoGen , CL264.. SEB , Sigma-Aldrich , S4881.SEB , Sigma-Aldrich , S4881.

    Article Title: Bacille Calmette–Guérin vaccine reprograms human neonatal lipid metabolism in vivo and in vitro
    Article Snippet: (LPC) kit , MyBioSource , Cat#: MBS2700657. .. TLR 7/8 agonist , Invivogen , R848 (Resiquimod) Cat#: tlrl-r848. .. Methanol , Applied Biosystems, ThermoFisher , Cat#: 400470.

    Article Title: Dendritic cell maturation, but not type I interferon exposure, restricts infection by HTLV-1, and viral transmission to T-cells
    Article Snippet: Chlorpromazine (20 μM, resuspended in H 2 O), diphenyl iodonium chloride (10 μM, resuspended in DMSO); dynasore (100 μM, resuspended in DMSO), methyl-β-cyclodextrin (10 mM, resuspended in H 2 O), nystatin (270 μM, resuspended in DMSO), amiloride (375 μM, resuspended in H 2 O), latrunculin (2 μM, resuspended in DMSO), cytochalasin D (2 μM, resuspended in DMSO), genistein (200 μM, resuspended in MeOH), wortmanin (40 μM, resuspended in DMSO), rottlerin (25 μM, resuspended in EtOH), Gö6953 (1 μM, resuspended in DMSO), NSC23766 trihydrochloride (250 μM, resuspended in H 2 O) and IPA-3 (125 μM, resuspended in DMSO) were purchased from Sigma.cytochalasin D (2 μM, resuspended in DMSO), genistein (200 μM, resuspended in MeOH), wortmanin (40 μM, resuspended in DMSO), rottlerin (25 μM, resuspended in EtOH), Gö6953 (1 μM, resuspended in DMSO), NSC23766 trihydrochloride (250 μM, resuspended in H 2 O) and IPA-3 (125 μM, resuspended in DMSO) were purchased from Sigma. ... Toll like receptor (TLR)-2 agonist (PAM3CSK4; 1 μg/ml); TLR-5 agonist (Flagellin; 200 ng/ml); TLR-3 agonist (Poly(I:C); 10 μg/ml); TLR-4 agonist (LPS; 1 μg/ml); TLR-7/8 agonist (R848; 3 μg/ml) were purchased from Invivogen.. Recombinant IFN-α (500–2000 IU/ml) was purchased from Tebu-Bio.Recombinant IFN-α (500–2000 IU/ml) was purchased from Tebu-Bio.

    Saline:

    Article Title: Peculiar transcriptional reprogramming with functional impairment of dendritic cells upon exposure to transformed HTLV-1-infected cells
    Article Snippet: Toll-like receptor (TLR)-4 agonist (LPS, tlrl-3pelps; 1μg/mL) and TLR-7/8 agonist (R848, tlrl-r848; 3μg/mL) were purchased from Invivogen.. MeV IC323-eGFP [ ] is a recombinant MeV expressing the gene-encoding eGFP (using the plasmid-encoding MeV IC323-eGFP kindly provided by Yanagi, Kyushu University, Fukuoka, Japan).MeV IC323-eGFP [ ] is a recombinant MeV expressing the gene-encoding eGFP (using the plasmid-encoding MeV IC323-eGFP kindly provided by Yanagi, Kyushu University, Fukuoka, Japan).

    Article Title: Induction of tolerogenicity following a molecular dialogue between HTLV-1-infected T cells and dendritic cells
    Article Snippet: Every experiment was repeated on MDDCs derived from independent blood donors.Every experiment was repeated on MDDCs derived from independent blood donors.. Reagents and antibodies Toll-like receptor (TLR)-4 agonist (LPS, tlrl-3pelps; 1µg/mL) and TLR-7/8 agonist (R848, tlrl-r848; 3µg/mL) were purchased from Invivogen.. MeV IC323-eGFP 48 is a recombinant MeV expressing the geneencoding eGFP (using the plasmid-encoding MeV IC323-eGFP kindly provided by Yanagi, Kyushu University, Fukuoka, Japan).MeV IC323-eGFP 48 is a recombinant MeV expressing the geneencoding eGFP (using the plasmid-encoding MeV IC323-eGFP kindly provided by Yanagi, Kyushu University, Fukuoka, Japan).

    Article Title: Lymph node dendritic cells harbor inducible replication-competent HIV despite years of suppressive ART
    Article Snippet: HIV peptides , JPT Peptide Technology , Customized.HIV peptides , JPT Peptide Technology , Customized.. TLR-7/8 agonist , InvivoGen , CL264.. SEB , Sigma-Aldrich , S4881.SEB , Sigma-Aldrich , S4881.

    Article Title: Bacille Calmette–Guérin vaccine reprograms human neonatal lipid metabolism in vivo and in vitro
    Article Snippet: (LPC) kit , MyBioSource , Cat#: MBS2700657. .. TLR 7/8 agonist , Invivogen , R848 (Resiquimod) Cat#: tlrl-r848. .. Methanol , Applied Biosystems, ThermoFisher , Cat#: 400470.

    Article Title: Dendritic cell maturation, but not type I interferon exposure, restricts infection by HTLV-1, and viral transmission to T-cells
    Article Snippet: Chlorpromazine (20 μM, resuspended in H 2 O), diphenyl iodonium chloride (10 μM, resuspended in DMSO); dynasore (100 μM, resuspended in DMSO), methyl-β-cyclodextrin (10 mM, resuspended in H 2 O), nystatin (270 μM, resuspended in DMSO), amiloride (375 μM, resuspended in H 2 O), latrunculin (2 μM, resuspended in DMSO), cytochalasin D (2 μM, resuspended in DMSO), genistein (200 μM, resuspended in MeOH), wortmanin (40 μM, resuspended in DMSO), rottlerin (25 μM, resuspended in EtOH), Gö6953 (1 μM, resuspended in DMSO), NSC23766 trihydrochloride (250 μM, resuspended in H 2 O) and IPA-3 (125 μM, resuspended in DMSO) were purchased from Sigma.cytochalasin D (2 μM, resuspended in DMSO), genistein (200 μM, resuspended in MeOH), wortmanin (40 μM, resuspended in DMSO), rottlerin (25 μM, resuspended in EtOH), Gö6953 (1 μM, resuspended in DMSO), NSC23766 trihydrochloride (250 μM, resuspended in H 2 O) and IPA-3 (125 μM, resuspended in DMSO) were purchased from Sigma. ... Toll like receptor (TLR)-2 agonist (PAM3CSK4; 1 μg/ml); TLR-5 agonist (Flagellin; 200 ng/ml); TLR-3 agonist (Poly(I:C); 10 μg/ml); TLR-4 agonist (LPS; 1 μg/ml); TLR-7/8 agonist (R848; 3 μg/ml) were purchased from Invivogen.. Recombinant IFN-α (500–2000 IU/ml) was purchased from Tebu-Bio.Recombinant IFN-α (500–2000 IU/ml) was purchased from Tebu-Bio.

    Purification:

    Article Title: Peculiar transcriptional reprogramming with functional impairment of dendritic cells upon exposure to transformed HTLV-1-infected cells
    Article Snippet: Toll-like receptor (TLR)-4 agonist (LPS, tlrl-3pelps; 1μg/mL) and TLR-7/8 agonist (R848, tlrl-r848; 3μg/mL) were purchased from Invivogen.. MeV IC323-eGFP [ ] is a recombinant MeV expressing the gene-encoding eGFP (using the plasmid-encoding MeV IC323-eGFP kindly provided by Yanagi, Kyushu University, Fukuoka, Japan).MeV IC323-eGFP [ ] is a recombinant MeV expressing the gene-encoding eGFP (using the plasmid-encoding MeV IC323-eGFP kindly provided by Yanagi, Kyushu University, Fukuoka, Japan).

    Article Title: Induction of tolerogenicity following a molecular dialogue between HTLV-1-infected T cells and dendritic cells
    Article Snippet: Every experiment was repeated on MDDCs derived from independent blood donors.Every experiment was repeated on MDDCs derived from independent blood donors.. Reagents and antibodies Toll-like receptor (TLR)-4 agonist (LPS, tlrl-3pelps; 1µg/mL) and TLR-7/8 agonist (R848, tlrl-r848; 3µg/mL) were purchased from Invivogen.. MeV IC323-eGFP 48 is a recombinant MeV expressing the geneencoding eGFP (using the plasmid-encoding MeV IC323-eGFP kindly provided by Yanagi, Kyushu University, Fukuoka, Japan).MeV IC323-eGFP 48 is a recombinant MeV expressing the geneencoding eGFP (using the plasmid-encoding MeV IC323-eGFP kindly provided by Yanagi, Kyushu University, Fukuoka, Japan).

    Article Title: Lymph node dendritic cells harbor inducible replication-competent HIV despite years of suppressive ART
    Article Snippet: HIV peptides , JPT Peptide Technology , Customized.HIV peptides , JPT Peptide Technology , Customized.. TLR-7/8 agonist , InvivoGen , CL264.. SEB , Sigma-Aldrich , S4881.SEB , Sigma-Aldrich , S4881.

    Article Title: Bacille Calmette–Guérin vaccine reprograms human neonatal lipid metabolism in vivo and in vitro
    Article Snippet: (LPC) kit , MyBioSource , Cat#: MBS2700657. .. TLR 7/8 agonist , Invivogen , R848 (Resiquimod) Cat#: tlrl-r848. .. Methanol , Applied Biosystems, ThermoFisher , Cat#: 400470.

    Article Title: Dendritic cell maturation, but not type I interferon exposure, restricts infection by HTLV-1, and viral transmission to T-cells
    Article Snippet: Chlorpromazine (20 μM, resuspended in H 2 O), diphenyl iodonium chloride (10 μM, resuspended in DMSO); dynasore (100 μM, resuspended in DMSO), methyl-β-cyclodextrin (10 mM, resuspended in H 2 O), nystatin (270 μM, resuspended in DMSO), amiloride (375 μM, resuspended in H 2 O), latrunculin (2 μM, resuspended in DMSO), cytochalasin D (2 μM, resuspended in DMSO), genistein (200 μM, resuspended in MeOH), wortmanin (40 μM, resuspended in DMSO), rottlerin (25 μM, resuspended in EtOH), Gö6953 (1 μM, resuspended in DMSO), NSC23766 trihydrochloride (250 μM, resuspended in H 2 O) and IPA-3 (125 μM, resuspended in DMSO) were purchased from Sigma.cytochalasin D (2 μM, resuspended in DMSO), genistein (200 μM, resuspended in MeOH), wortmanin (40 μM, resuspended in DMSO), rottlerin (25 μM, resuspended in EtOH), Gö6953 (1 μM, resuspended in DMSO), NSC23766 trihydrochloride (250 μM, resuspended in H 2 O) and IPA-3 (125 μM, resuspended in DMSO) were purchased from Sigma. ... Toll like receptor (TLR)-2 agonist (PAM3CSK4; 1 μg/ml); TLR-5 agonist (Flagellin; 200 ng/ml); TLR-3 agonist (Poly(I:C); 10 μg/ml); TLR-4 agonist (LPS; 1 μg/ml); TLR-7/8 agonist (R848; 3 μg/ml) were purchased from Invivogen.. Recombinant IFN-α (500–2000 IU/ml) was purchased from Tebu-Bio.Recombinant IFN-α (500–2000 IU/ml) was purchased from Tebu-Bio.

    Luminex:

    Article Title: Peculiar transcriptional reprogramming with functional impairment of dendritic cells upon exposure to transformed HTLV-1-infected cells
    Article Snippet: Toll-like receptor (TLR)-4 agonist (LPS, tlrl-3pelps; 1μg/mL) and TLR-7/8 agonist (R848, tlrl-r848; 3μg/mL) were purchased from Invivogen.. MeV IC323-eGFP [ ] is a recombinant MeV expressing the gene-encoding eGFP (using the plasmid-encoding MeV IC323-eGFP kindly provided by Yanagi, Kyushu University, Fukuoka, Japan).MeV IC323-eGFP [ ] is a recombinant MeV expressing the gene-encoding eGFP (using the plasmid-encoding MeV IC323-eGFP kindly provided by Yanagi, Kyushu University, Fukuoka, Japan).

    Article Title: Induction of tolerogenicity following a molecular dialogue between HTLV-1-infected T cells and dendritic cells
    Article Snippet: Every experiment was repeated on MDDCs derived from independent blood donors.Every experiment was repeated on MDDCs derived from independent blood donors.. Reagents and antibodies Toll-like receptor (TLR)-4 agonist (LPS, tlrl-3pelps; 1µg/mL) and TLR-7/8 agonist (R848, tlrl-r848; 3µg/mL) were purchased from Invivogen.. MeV IC323-eGFP 48 is a recombinant MeV expressing the geneencoding eGFP (using the plasmid-encoding MeV IC323-eGFP kindly provided by Yanagi, Kyushu University, Fukuoka, Japan).MeV IC323-eGFP 48 is a recombinant MeV expressing the geneencoding eGFP (using the plasmid-encoding MeV IC323-eGFP kindly provided by Yanagi, Kyushu University, Fukuoka, Japan).

    Article Title: Lymph node dendritic cells harbor inducible replication-competent HIV despite years of suppressive ART
    Article Snippet: HIV peptides , JPT Peptide Technology , Customized.HIV peptides , JPT Peptide Technology , Customized.. TLR-7/8 agonist , InvivoGen , CL264.. SEB , Sigma-Aldrich , S4881.SEB , Sigma-Aldrich , S4881.

    Article Title: Bacille Calmette–Guérin vaccine reprograms human neonatal lipid metabolism in vivo and in vitro
    Article Snippet: (LPC) kit , MyBioSource , Cat#: MBS2700657. .. TLR 7/8 agonist , Invivogen , R848 (Resiquimod) Cat#: tlrl-r848. .. Methanol , Applied Biosystems, ThermoFisher , Cat#: 400470.

    Article Title: Dendritic cell maturation, but not type I interferon exposure, restricts infection by HTLV-1, and viral transmission to T-cells
    Article Snippet: Chlorpromazine (20 μM, resuspended in H 2 O), diphenyl iodonium chloride (10 μM, resuspended in DMSO); dynasore (100 μM, resuspended in DMSO), methyl-β-cyclodextrin (10 mM, resuspended in H 2 O), nystatin (270 μM, resuspended in DMSO), amiloride (375 μM, resuspended in H 2 O), latrunculin (2 μM, resuspended in DMSO), cytochalasin D (2 μM, resuspended in DMSO), genistein (200 μM, resuspended in MeOH), wortmanin (40 μM, resuspended in DMSO), rottlerin (25 μM, resuspended in EtOH), Gö6953 (1 μM, resuspended in DMSO), NSC23766 trihydrochloride (250 μM, resuspended in H 2 O) and IPA-3 (125 μM, resuspended in DMSO) were purchased from Sigma.cytochalasin D (2 μM, resuspended in DMSO), genistein (200 μM, resuspended in MeOH), wortmanin (40 μM, resuspended in DMSO), rottlerin (25 μM, resuspended in EtOH), Gö6953 (1 μM, resuspended in DMSO), NSC23766 trihydrochloride (250 μM, resuspended in H 2 O) and IPA-3 (125 μM, resuspended in DMSO) were purchased from Sigma. ... Toll like receptor (TLR)-2 agonist (PAM3CSK4; 1 μg/ml); TLR-5 agonist (Flagellin; 200 ng/ml); TLR-3 agonist (Poly(I:C); 10 μg/ml); TLR-4 agonist (LPS; 1 μg/ml); TLR-7/8 agonist (R848; 3 μg/ml) were purchased from Invivogen.. Recombinant IFN-α (500–2000 IU/ml) was purchased from Tebu-Bio.Recombinant IFN-α (500–2000 IU/ml) was purchased from Tebu-Bio.

    In Vivo:

    Article Title: Peculiar transcriptional reprogramming with functional impairment of dendritic cells upon exposure to transformed HTLV-1-infected cells
    Article Snippet: Toll-like receptor (TLR)-4 agonist (LPS, tlrl-3pelps; 1μg/mL) and TLR-7/8 agonist (R848, tlrl-r848; 3μg/mL) were purchased from Invivogen.. MeV IC323-eGFP [ ] is a recombinant MeV expressing the gene-encoding eGFP (using the plasmid-encoding MeV IC323-eGFP kindly provided by Yanagi, Kyushu University, Fukuoka, Japan).MeV IC323-eGFP [ ] is a recombinant MeV expressing the gene-encoding eGFP (using the plasmid-encoding MeV IC323-eGFP kindly provided by Yanagi, Kyushu University, Fukuoka, Japan).

    Article Title: Induction of tolerogenicity following a molecular dialogue between HTLV-1-infected T cells and dendritic cells
    Article Snippet: Every experiment was repeated on MDDCs derived from independent blood donors.Every experiment was repeated on MDDCs derived from independent blood donors.. Reagents and antibodies Toll-like receptor (TLR)-4 agonist (LPS, tlrl-3pelps; 1µg/mL) and TLR-7/8 agonist (R848, tlrl-r848; 3µg/mL) were purchased from Invivogen.. MeV IC323-eGFP 48 is a recombinant MeV expressing the geneencoding eGFP (using the plasmid-encoding MeV IC323-eGFP kindly provided by Yanagi, Kyushu University, Fukuoka, Japan).MeV IC323-eGFP 48 is a recombinant MeV expressing the geneencoding eGFP (using the plasmid-encoding MeV IC323-eGFP kindly provided by Yanagi, Kyushu University, Fukuoka, Japan).

    Article Title: Lymph node dendritic cells harbor inducible replication-competent HIV despite years of suppressive ART
    Article Snippet: HIV peptides , JPT Peptide Technology , Customized.HIV peptides , JPT Peptide Technology , Customized.. TLR-7/8 agonist , InvivoGen , CL264.. SEB , Sigma-Aldrich , S4881.SEB , Sigma-Aldrich , S4881.

    Article Title: Bacille Calmette–Guérin vaccine reprograms human neonatal lipid metabolism in vivo and in vitro
    Article Snippet: (LPC) kit , MyBioSource , Cat#: MBS2700657. .. TLR 7/8 agonist , Invivogen , R848 (Resiquimod) Cat#: tlrl-r848. .. Methanol , Applied Biosystems, ThermoFisher , Cat#: 400470.

    Article Title: Dendritic cell maturation, but not type I interferon exposure, restricts infection by HTLV-1, and viral transmission to T-cells
    Article Snippet: Chlorpromazine (20 μM, resuspended in H 2 O), diphenyl iodonium chloride (10 μM, resuspended in DMSO); dynasore (100 μM, resuspended in DMSO), methyl-β-cyclodextrin (10 mM, resuspended in H 2 O), nystatin (270 μM, resuspended in DMSO), amiloride (375 μM, resuspended in H 2 O), latrunculin (2 μM, resuspended in DMSO), cytochalasin D (2 μM, resuspended in DMSO), genistein (200 μM, resuspended in MeOH), wortmanin (40 μM, resuspended in DMSO), rottlerin (25 μM, resuspended in EtOH), Gö6953 (1 μM, resuspended in DMSO), NSC23766 trihydrochloride (250 μM, resuspended in H 2 O) and IPA-3 (125 μM, resuspended in DMSO) were purchased from Sigma.cytochalasin D (2 μM, resuspended in DMSO), genistein (200 μM, resuspended in MeOH), wortmanin (40 μM, resuspended in DMSO), rottlerin (25 μM, resuspended in EtOH), Gö6953 (1 μM, resuspended in DMSO), NSC23766 trihydrochloride (250 μM, resuspended in H 2 O) and IPA-3 (125 μM, resuspended in DMSO) were purchased from Sigma. ... Toll like receptor (TLR)-2 agonist (PAM3CSK4; 1 μg/ml); TLR-5 agonist (Flagellin; 200 ng/ml); TLR-3 agonist (Poly(I:C); 10 μg/ml); TLR-4 agonist (LPS; 1 μg/ml); TLR-7/8 agonist (R848; 3 μg/ml) were purchased from Invivogen.. Recombinant IFN-α (500–2000 IU/ml) was purchased from Tebu-Bio.Recombinant IFN-α (500–2000 IU/ml) was purchased from Tebu-Bio.

    Software:

    Article Title: Peculiar transcriptional reprogramming with functional impairment of dendritic cells upon exposure to transformed HTLV-1-infected cells
    Article Snippet: Toll-like receptor (TLR)-4 agonist (LPS, tlrl-3pelps; 1μg/mL) and TLR-7/8 agonist (R848, tlrl-r848; 3μg/mL) were purchased from Invivogen.. MeV IC323-eGFP [ ] is a recombinant MeV expressing the gene-encoding eGFP (using the plasmid-encoding MeV IC323-eGFP kindly provided by Yanagi, Kyushu University, Fukuoka, Japan).MeV IC323-eGFP [ ] is a recombinant MeV expressing the gene-encoding eGFP (using the plasmid-encoding MeV IC323-eGFP kindly provided by Yanagi, Kyushu University, Fukuoka, Japan).

    Article Title: Induction of tolerogenicity following a molecular dialogue between HTLV-1-infected T cells and dendritic cells
    Article Snippet: Every experiment was repeated on MDDCs derived from independent blood donors.Every experiment was repeated on MDDCs derived from independent blood donors.. Reagents and antibodies Toll-like receptor (TLR)-4 agonist (LPS, tlrl-3pelps; 1µg/mL) and TLR-7/8 agonist (R848, tlrl-r848; 3µg/mL) were purchased from Invivogen.. MeV IC323-eGFP 48 is a recombinant MeV expressing the geneencoding eGFP (using the plasmid-encoding MeV IC323-eGFP kindly provided by Yanagi, Kyushu University, Fukuoka, Japan).MeV IC323-eGFP 48 is a recombinant MeV expressing the geneencoding eGFP (using the plasmid-encoding MeV IC323-eGFP kindly provided by Yanagi, Kyushu University, Fukuoka, Japan).

    Article Title: Lymph node dendritic cells harbor inducible replication-competent HIV despite years of suppressive ART
    Article Snippet: HIV peptides , JPT Peptide Technology , Customized.HIV peptides , JPT Peptide Technology , Customized.. TLR-7/8 agonist , InvivoGen , CL264.. SEB , Sigma-Aldrich , S4881.SEB , Sigma-Aldrich , S4881.

    Article Title: Bacille Calmette–Guérin vaccine reprograms human neonatal lipid metabolism in vivo and in vitro
    Article Snippet: (LPC) kit , MyBioSource , Cat#: MBS2700657. .. TLR 7/8 agonist , Invivogen , R848 (Resiquimod) Cat#: tlrl-r848. .. Methanol , Applied Biosystems, ThermoFisher , Cat#: 400470.

    Article Title: Dendritic cell maturation, but not type I interferon exposure, restricts infection by HTLV-1, and viral transmission to T-cells
    Article Snippet: Chlorpromazine (20 μM, resuspended in H 2 O), diphenyl iodonium chloride (10 μM, resuspended in DMSO); dynasore (100 μM, resuspended in DMSO), methyl-β-cyclodextrin (10 mM, resuspended in H 2 O), nystatin (270 μM, resuspended in DMSO), amiloride (375 μM, resuspended in H 2 O), latrunculin (2 μM, resuspended in DMSO), cytochalasin D (2 μM, resuspended in DMSO), genistein (200 μM, resuspended in MeOH), wortmanin (40 μM, resuspended in DMSO), rottlerin (25 μM, resuspended in EtOH), Gö6953 (1 μM, resuspended in DMSO), NSC23766 trihydrochloride (250 μM, resuspended in H 2 O) and IPA-3 (125 μM, resuspended in DMSO) were purchased from Sigma.cytochalasin D (2 μM, resuspended in DMSO), genistein (200 μM, resuspended in MeOH), wortmanin (40 μM, resuspended in DMSO), rottlerin (25 μM, resuspended in EtOH), Gö6953 (1 μM, resuspended in DMSO), NSC23766 trihydrochloride (250 μM, resuspended in H 2 O) and IPA-3 (125 μM, resuspended in DMSO) were purchased from Sigma. ... Toll like receptor (TLR)-2 agonist (PAM3CSK4; 1 μg/ml); TLR-5 agonist (Flagellin; 200 ng/ml); TLR-3 agonist (Poly(I:C); 10 μg/ml); TLR-4 agonist (LPS; 1 μg/ml); TLR-7/8 agonist (R848; 3 μg/ml) were purchased from Invivogen.. Recombinant IFN-α (500–2000 IU/ml) was purchased from Tebu-Bio.Recombinant IFN-α (500–2000 IU/ml) was purchased from Tebu-Bio.



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    Image Search Results


    a. Schematic representation of the experimental design. MDDCs were exposed to uninfected (Jurkat, green) or HTLV-1-infected T cells (C91-PL, blue) for 24h, before restimulation with LPS or R848 for an additional 24h. b. Flow cytometry analysis after CD11c and CD86 staining, in LPS- ( left ) or R848-restimulated MDDCs ( right ) pre-exposed to Jurkat (red) or C91-PL (dark blue) cells. Data are represented as the normalized MFI of CD86, with the MFI in restimulated Jurkat-pre-exposed MDDCs set to 100 (n = 30 or 10 independent experiments, respectively). Presented data are a subset of , respectively and were analysed with Kruskal-Wallis test or ordinary one-way ANOVA, respectively as described in and Tables c. Supernatant from the indicated cocultures was collected after LPS restimulation, and TNF-α ( left ) and IFN-I ( right ) concentrations were quantified for n = 3 or 10 independent experiments, respectively. Presented data are a subset of and were analysed with RM one-way ANOVA or ordinary one-way ANOVA, respectively as described in and Tables. d. Schematic drawing summarizing the results from Figs and . The drawing was created using BioRender.com .

    Journal: PLOS Pathogens

    Article Title: Peculiar transcriptional reprogramming with functional impairment of dendritic cells upon exposure to transformed HTLV-1-infected cells

    doi: 10.1371/journal.ppat.1012555

    Figure Lengend Snippet: a. Schematic representation of the experimental design. MDDCs were exposed to uninfected (Jurkat, green) or HTLV-1-infected T cells (C91-PL, blue) for 24h, before restimulation with LPS or R848 for an additional 24h. b. Flow cytometry analysis after CD11c and CD86 staining, in LPS- ( left ) or R848-restimulated MDDCs ( right ) pre-exposed to Jurkat (red) or C91-PL (dark blue) cells. Data are represented as the normalized MFI of CD86, with the MFI in restimulated Jurkat-pre-exposed MDDCs set to 100 (n = 30 or 10 independent experiments, respectively). Presented data are a subset of , respectively and were analysed with Kruskal-Wallis test or ordinary one-way ANOVA, respectively as described in and Tables c. Supernatant from the indicated cocultures was collected after LPS restimulation, and TNF-α ( left ) and IFN-I ( right ) concentrations were quantified for n = 3 or 10 independent experiments, respectively. Presented data are a subset of and were analysed with RM one-way ANOVA or ordinary one-way ANOVA, respectively as described in and Tables. d. Schematic drawing summarizing the results from Figs and . The drawing was created using BioRender.com .

    Article Snippet: Toll-like receptor (TLR)-4 agonist (LPS, tlrl-3pelps; 1μg/mL) and TLR-7/8 agonist (R848, tlrl-r848; 3μg/mL) were purchased from Invivogen.

    Techniques: Infection, Flow Cytometry, Staining